Description
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated FGF-23 concentrations in Porcine serum, plasma and other body fluids. Using Purified Porcine FGF-23 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add FGF-23 and FGF-23 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of FGF-23 in the samples is then determined by comparing the O.D. of the samples to the standard curve